Comparison of Estradiol Cypionate, Estradiol Benzoate and Gonadotropin-Releasing Hormone in Fixed-Time Artificial Insemination in Multiparous Murrah Buffaloes

The present study was aimed to evaluate the efficiency of estradiol cypionate, benzoate and gonadotropin-releasing hormone during fixed-time artificial insemination (FTAI) protocols in multiparous Murrah buffaloes. A total 35 buffaloes were used between September November 2021 subjected a synchronization protocol. On day 0, insertion an intravaginal progesterone device (CIDR, 1.38 g) made with intramuscular (i/m) injection (EB, 2 mg); 9: CIDR removed i/m injections Dinoprost (12.5 mg) eCG (400 IU), then randomized into three treatment group: 1) EB 1 mg on 10 (n=13). 2) cypionate (EC) 9 (n=12), 3) Buserelin acetate (GnRH) 0.0105 (n=10). estrus signs observed FTAI (56 h after withdrawal) conception rate at 90 days recorded. rates 61.5, 58.3 60.0% for EB, EC GnRH groups, respectively. There no significant difference groups estrus. Therefore, it concluded that all treatments can be efficiently protocol